A general assay for antibody catalysis using acridone as a fluorescent tag.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A general assay for antibody catalysis using acridone as a fluorescent tag.

A simple and highly sensitive catalysis assay is demonstrated based on analyzing reactions with acridonetagged compounds by thin-layer chromatography. As little as 1 pmol of product is readily visualized by its blue fluorescence under UV illumination and identified by its retention factor (Rf). Each assay requires only 10 microliters of solution. The method is reliable, inexpensive, versatile, ...

متن کامل

Inclusion fluorescent-antibody test as a screening assay for detection of antibodies to Chlamydia pneumoniae.

A study was conducted to determine the ability of the inclusion immunofluorescence assay (inclusion IFA) to act as a screening test to detect samples with antibodies to Chlamydia pneumoniae; microimmunofluorescence (MIF) was used as the "gold standard." In addition, the inclusion IFA was compared using HEp-2 cells infected with either C. pneumoniae CM-1 or Chlamydia trachomatis serovar E. A tot...

متن کامل

3 - Propiolactone Decontamination of Simian Virus - 40 as Determined by a Rapid Fluorescent - Antibody Assay

LEVINE, S. I. (The National Drug Co., Philadelphia, Pa.), N. R. GOULET, AND 0. C. Liu. ,B-Propiolactone decontamination of Simian virus-40 as determined by a rapid fluorescent-antibody assay. Appl. Microbiol. 13:70-72. 1965.-,3-Propiolactone vapor treatment of vaccine production facilities has been shown to be approximately 90%o effective in the elimination of large quantities of Simian virus-4...

متن کامل

Beta-propiolactone Decontamination of Simian Virus-40 as Determined by a Rapid Fluorescent-antibody Assay.

beta-Propiolactone vapor treatment of vaccine production facilities has been shown to be approximately 90% effective in the elimination of large quantities of Simian virus-40 (SV(40)). The use of a rapid fluorescent-antibody assay for the detection of SV(40) was also studied.

متن کامل

evaluation of confounders in toxoplasmosis indirect fluorescent antibody assay

background : the ifa test is one of the most usual methods for detecting anti- toxoplasma antibod­ies, although it has not any unique standardization. it seems that the microscopic judg­ment of results is an important confounder in ifa test. therefore, we conducted the present study to clarify the role of microscopic observer, and other confounders on the test. methods: eighty sera were collect...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1996

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.93.9.4251